Cloning Services

Complete molecular cloning solutions from design to delivery

7-10 Day Turnaround • Consultation Required

Expert Cloning for Biotech and Pharma

Our Cloning Services provide end-to-end molecular cloning solutions backed by 20+ years of drug discovery experience. From simple subcloning to complex multi-fragment assemblies, we deliver sequence-verified constructs on time, every time.

We specialize in challenging projects that others can't handle—GC-rich sequences, repetitive elements, toxic genes, and complex multi-component systems. Our proprietary methods cut delivery times in half while maintaining 100% sequence accuracy.

All cloning projects start with a consultation to ensure we understand your goals and design the optimal approach. Whether you need a single construct or a library of thousands, we're your local partner for reliable molecular cloning.

Quick Facts

Standard Turnaround
7-10 days
Vector Support
Any Backbone
Quality
100% Verified
Guarantee
On-Time or Free

Our Cloning Capabilities

Comprehensive services for every cloning need

Gene Synthesis & Sub-cloning ≤10 days

De-novo synthesis up to 1 kb with seamless insertion into any vector. Codon-optimized and error-proofed using our trade-secret methods that cut delivery time in half.

Any backbone Codon optimization Error-proofed

Multi-Fragment Assembly ≤10 days

Gibson/NEBuilder assembly of 2-6 fragments into vectors up to 14 kb total. We handle complex assemblies including multi-insert into separate sites.

Up to 6 fragments 14 kb max size GC rescue methods

Site-Directed Mutagenesis ≤10 days

Precise point, deletion, and insertion mutations with our low-fail rate workflow. Price scales with variant count for efficient SAR campaigns.

Point mutations Deletions Insertions

DNA Prep & QC 24-72h

Mini to Giga scale preparations with full sequence verification. Endotoxin-free options available for cell culture and pre-clinical applications.

Mini to Giga Endotoxin-free NGS confirmation

Vector/Library Construction Quote-based

Bar-coded pooled or arrayed libraries for lentiviral, AAV, baculoviral, and CRISPR systems. Up to 25k constructs per pool with full representation validation.

Lenti/AAV/Baculo CRISPR libraries Up to 25k/pool

Design & Consultation Quote-based

Expert guidance for challenging projects. Codon optimization, troubleshooting difficult sequences, and comprehensive project planning from our experienced team.

Codon optimization Troubleshooting Project scoping

📞 All Cloning Services Begin with Consultation

We'll discuss your project, recommend the optimal approach, and provide a detailed quote and timeline.

Request Consultation

Why Choose Vectide for Cloning?

Speed

7-10 day delivery with proprietary methods that cut typical timelines in half

🎯

Expertise

20+ years experience including work on FDA-approved therapeutics

Quality

100% sequence verification and comprehensive QC documentation

📍

Local

Boston-based lab with in-person support and consultation

Universal Backbone Compatibility

We work with any vector system you need

Bacterial Vectors
Lentiviral
Retroviral
AAV
Baculoviral
Yeast Vectors

Common Applications

Supporting research across biotech and pharma

Therapeutic Development

Construct generation for biologics, gene therapy vectors, and cell therapy programs with pre-clinical quality requirements.

Protein Engineering

Expression constructs, domain shuffling, and variant libraries for enzyme optimization and antibody engineering.

Functional Genomics

CRISPR libraries, ORF collections, and screening constructs for target identification and validation.

Assay Development

Reporter constructs, biosensors, and tool reagents for high-throughput screening and diagnostic development.

Ready to Start Your Cloning Project?

Let's discuss your needs and design the optimal approach