Complex Gibson/NEBuilder assemblies with up to 6 fragments
Our Multi-Fragment Assembly service handles the most challenging cloning projects with 2-6 Gibson/NEBuilder fragments assembled into vectors up to 14kb total size.
We specialize in complicated assemblies including multi-insert constructions into separate insertion sites. Our trade-secret GC extremes rescue method ensures success even with challenging sequences like CAG repeats.
Perfect for reshuffling vectors and inserts, creating complex expression cassettes, or building multi-component systems in a single construct.
Reshuffling vectors and inserts for complex constructs
Construct polycistronic vectors with multiple genes, promoters, and regulatory elements for co-expression studies or metabolic pathway engineering.
Combine functional domains from different proteins to create chimeric proteins with novel properties for therapeutic or research applications.
Build multi-component reporter constructs with different fluorescent proteins, selection markers, and regulatory elements in a single vector.
Assemble entire biosynthetic pathways by combining multiple enzyme-coding sequences with appropriate regulatory elements for metabolic engineering.
Get your constructs delivered on time, guaranteed