NanoPore Sequencing

Long-read sequencing for complete plasmid coverage in a single read

24-48 Hour Turnaround

Complete Sequence Coverage with Long Reads

Our NanoPore Sequencing service uses Oxford Nanopore technology to deliver complete sequence coverage of your constructs in a single read. No more stitching together multiple Sanger reactions—get the full picture with one sequencing run.

NanoPore's long-read capability is ideal for whole plasmid verification, where you need to confirm the entire construct including backbone, insert, and all junctions. It's also perfect for large PCR products and situations where Sanger's read length limitations become a bottleneck.

With 24-48 hour turnaround from our local Cambridge lab, you can verify complex constructs quickly and move forward with confidence in your experimental timeline.

Quick Facts

Turnaround
24-48 hours
Price
$15 per sample
Read Length
Up to 20+ kb
Coverage
Full Plasmid
Location
Cambridge, MA

Service Options

Choose the right NanoPore service for your needs

Whole Plasmid

Most Popular

Complete plasmid sequencing in a single read. Verify your entire construct including backbone, insert, promoters, and all junctions.

  • Full coverage up to 20+ kb
  • No primer design needed
  • Detect rearrangements
  • Confirm all elements

PCR Products

Long Amplicons

Sequence PCR products that exceed Sanger's read length. Ideal for long-range PCR verification and amplicon sequencing.

  • Products up to 10+ kb
  • No cloning required
  • Direct from PCR
  • Size flexibility

Colony Sequencing

High-Throughput

Screen colonies directly without plasmid prep. Get full-length sequence data to identify correct clones quickly.

  • Skip miniprep step
  • Full construct verification
  • 96-well plate format
  • Cost-effective screening

Service Specifications

🧬 What We Accept

  • Purified plasmid DNA (50-100 ng/μL)
  • Purified PCR products
  • Bacterial colonies or cultures
  • Glycerol stocks
  • 96-well plate format available
  • Individual tubes for smaller batches
  • Download the NanoPore submission template

📊 What You Get

  • Consensus sequence (FASTA)
  • Raw read data (FASTQ)
  • Coverage and quality metrics
  • Alignment to reference (if provided)
  • Online data access via dashboard
  • Technical support included

When to Choose NanoPore

Long-read sequencing shines in these applications

Complex Constructs

Multi-fragment assemblies, large inserts, or constructs with repetitive elements that are difficult to verify with Sanger.

Backbone Verification

Confirm your entire vector including origin, resistance markers, and regulatory elements in addition to your insert.

Rearrangement Detection

Identify unexpected deletions, insertions, or rearrangements that might be missed with targeted Sanger reads.

Time-Sensitive Projects

When you need complete verification quickly without designing and running multiple Sanger reactions.

Ready to Start?

Get complete plasmid coverage with 24-48 hour turnaround