Sanger Sequencing

Reliable, cost-effective sequencing for everyday verification needs

24-48 Hour Turnaround

The Gold Standard for Routine Sequencing

Our Sanger Sequencing service delivers fast, accurate results for your everyday sequencing needs. Whether you're verifying a cloning construct, screening colonies, or confirming a mutation, Sanger sequencing remains the most cost-effective and reliable method for targeted sequence verification.

As a local Boston-area lab, we offer 24-48 hour turnaround for samples received by our morning cutoff. Drop off your samples in person or ship them to us—either way, you'll get high-quality sequence data with the personal service of a neighborhood lab.

Perfect for routine plasmid verification, insert confirmation, mutation screening, and any application where you need reliable sequence data from a specific region of your construct. Pricing starts at $5 per reaction.

Quick Facts

Turnaround
24-48 hours
Price
$5 per reaction
Read Length
Up to 1000 bp
Sample Types
Plasmid, PCR, Purified DNA
Location
Cambridge, MA

Service Specifications

🧬 What We Accept

  • Purified plasmid DNA (100-200 ng/μL)
  • PCR products (purified or unpurified)
  • Bacterial cultures or glycerol stocks
  • Your primers or use our universal primers
  • 96-well plate format for high-throughput
  • Individual tubes for smaller batches
  • Download the Sanger submission template

📊 What You Get

  • High-quality chromatogram files (.ab1)
  • FASTA sequence files
  • Quality scores and read length metrics
  • Online data access through your dashboard
  • Email notification when results are ready
  • Technical support for troubleshooting

Common Applications

Trusted by researchers for everyday sequencing needs

Plasmid Verification

Confirm your cloning worked correctly. Verify inserts, junctions, and critical regions before proceeding with experiments.

Colony Screening

Screen multiple colonies quickly and cost-effectively to identify correct clones from your transformation plates.

Mutation Confirmation

Verify site-directed mutagenesis results, confirm point mutations, and validate edited sequences.

PCR Product Verification

Confirm PCR amplification specificity and sequence accuracy before downstream applications.

Ready to Start?

Get 24-48 hour results from your local sequencing lab