Services Overview

7-10 business days

Cloning Services

• Gene synthesis & sub-cloning up to 1 kb
• Multi-fragment Gibson/NEBuilder assemblies
• Site-directed mutagenesis (point, deletion, insertion)

Popular add-ons: DNA prep & QC; vector/library construction

Turnaround: 7-10 days standard

Typical use: Sequence-verified constructs and libraries

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Consultation required

Next-Generation Sequencing (NGS)

• Illumina RNA-seq for expression profiling
• NanoPore long-read sequencing for WGS and structural variants
• Hybrid Illumina + NanoPore workflows

Popular add-ons: Experimental design support; data analysis

Turnaround: Quote-based

Typical use: Transcriptomics, WGS, and variant discovery

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24-48 hours

NanoPore Sequencing

• Whole plasmid sequencing in a single read
• Long PCR products and colony sequencing

Popular add-ons: Reference alignment and coverage reports

Turnaround: 24-48 hours

Typical use: Full construct verification

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Same-day results

Sanger Sequencing

• Plasmid verification and colony screening
• Routine mutation confirmation

Popular add-ons: Universal primers; 96-well format

Turnaround: Same-day

Typical use: Fast verification and screening

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24-72 h

DNA Prep & QC

• Mini → Midi → Maxi → Mega → Giga
• OD260/280; optional gel QC

Popular add-ons: NGS confirmation; endotoxin-free; ship-on-dry-ice

Turnaround: Mini 24 h; Midi+ 72 h

Typical use: Pre-clinical scale-up batches

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Quote-based

Vector / Library Construction

• Bar-coded pooled or arrayed libraries (lenti, AAV, baculo, CRISPR)
• Up to 25k constructs / pool

Popular add-ons: Titer-ready lenti/AAV prep; cell-bank generation

Turnaround: Quote-based (~1 month typical)

Typical use: Optimize gene expression & gene motif

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